MATERIAL DE MICROSCOPIA ELECTRÓNICA

Productos Químicos

Acetonitrile

Suggested substitute for Propylene Oxide because it is reported as noncarcinogenic, less toxic and not as flammable. High purity; miscible with water, alcohols, acetone and epoxy resins15.

Benzyldimethylamine

(BDMA) N,N-Dimethylbenzylamine Catalyst for epoxy resins. Has lower viscosity compared to DMP-30.

Cacodylic Acid, Sodium Salt, trihydrate

Arsenic compound. Highly toxic. Hygroscopic. Buffer.

Chloramine-T Algicide

A non-corrosive algicide for use with constant temperature bath or recirculation chillers like the PELCO Steadytemp™ and chillers used for TEM/SEM systems. Chloramine-T algicide prevents the growth of algae which can be damaging to equipment and instrumentation. The product can be used as a shock treatment, or as a preventative treatment at a dosage of 1 gram per gallon. Best to use distilled water to avoid scaling and shield the bath and cooling lines from light.

Formaldehyde

EM grade paraformaldehyde fixative and Histology Fixative. Paraformaldehyde solution (methanol free). Pre-scored amber ampules sealed under inert gas.

Formvar

Substrate Component.Polyvinyl formal powder. Substrates for EM; soluble in ethylene dichloride, chloroform, dioxane.

Glutaraldehyde

EM Fixative. EM grade purified glass distilled Glutaraldehyde sealed with inert gas in amber small-volume ampules, or in bottles for larger volumes.

LR White Resin

Resin with catalyst packaged separately. Accelerator can be used for cold-cure (room temperature). Heat cure is 24 hours between 50°C and 65°C. Gelatin capsules are recommended for temperatures below 60°C. Tightly closed BEEM® Capsules and PTFE Molds with ACLAR (tm) as cover  work well at 65°C. Widely used because of excellent immunolabeling results. LR White is a polar, monomer polyhydroxylated aromatic acrylic resin.
Sections from LR White appear "bumpy" in contrast to Epon resin sections. It is possible that Epon links covalently to tissue proteins while LR White links more weakly. "LR White appears to act in a manner similar to ice in a freeze-fractured specimen"

Methyl Methacrylate Monomer

An excellent negative stain. Unlike phosphotungstic Acid, it does not damage virus particles and it is consequently valuable for staining delicate viruses. For embedding very hard specimens

Methylamine Tungstate NMA

Anhydride . Methyl-5-norbornene-2,3-dicarboxylic anhydride. Moisture sensitive. Viscosity 225-275cp, density 1.24g/ml. Hardener for epoxy resins.

Osmium Tetroxide

EM Fixative. Solid vaporizes by sublimination. Should be used only under the hood. Affects eyes. Use eye protection.
EM Grade, glass ampules in black paper. No sticky labels. Osmium purity >99.95%. Light sensitive.

Paraffin

A mixture of fine paraffin waxes and copolymer alloys in convenient pellets. Produces ultra-thin compression-free ribbons. It is used to cut 2um sections routinely without icing. Functions flawlessly in pressure/vacuum fluid flow processors. Provides maximum support for both hard and soft tissue samples while maintaining exceptional clarity. Low melting point eliminates excessive heat distortion during tissue processing. Melting point 55°C.

Paraformaldehyde

EM/LM Fixative. Polyoxymethylene. White free-flowing solid prill with strong, pungent formaldehyde odor. Avoid strong oxidizing agents, temperature above 38°C.

Pioloform Powder

Substrate component. Water-insoluble. Good thermal and mechanical stability. Because of small mass thickness, electron scattering within the film is low. 0.3­1.5% solution in chloroform, filtered through filter paper.

Propylene Oxide

1,2-epoxypropane. Must use under hood, and with all caution. Extremely low vapor pressure. "Since epoxy resins are soluble in ethanol and acetone, dehydration is commonly carried out with one of these solvents. Because epoxy resins are more readily soluble in propylene oxide...this solvent is frequently used immediately prior to infiltration with the resin. ...most resins are not readily miscible with ethanol". Propylene Oxide does not separate from the epoxy resin during curing, thus small amounts left in the resin do no harm.< br />
Luft introduced Propylene Oxide into epoxy mixtures with his landmark paper and helped bring about improved embedding cures, better ultrastructural research results with blocks that would more reliably section and give better performance under the EM beam by reducing viscosity and increasing penetration.

Ruthenium Red

Fixative / Stain

Sodium Hydroxide

for making Paraformaldehyde and Lead Staining.

Sodium Phosphate, monobasic and dibasic

Buffer

Tissue-Tek

A mold release concentrate used to coat base molds for easy release of paraffin blocks. Dilute 5ml of concentrate with 95ml of ethyl or iso-propyl alcohol. Dip molds and dry before use. 4 oz. bottle.

Toluidine Blue

LM Stain. For epoxy sections: 0.5% toluidine blue in 0.1% sodium carbonate solution at pH 11.1
For methacrylate/acrylic sections: 0.05% toluidine blue in benzoate buffer pH 4.4 (benzoic acid 0.25g, sodium benzoate 0.29g, water 200ml),
p 5:3.11, "Procedures in Electron Microscopy"

Uranyl Acetate

Positive or negative stain, EM. May cause adverse mutagenic or teratogenic effect.

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